The human amniotic membrane (AM) is widely used in ophthalmic surgery worldwide. The list of indications for amniotic membrane transplantation (AMT) in ophthalmology continues to grow each year. There are three main techniques for amniotic membrane transplantation: onlay (biological coverage), inlay (multilayer transplantation), and sandwich (combined technique). However, there is no standard technique for AMT. Surgical trauma during AMT, caused by a large number of sutures, has prompted the development of improved methods.

Researchers from the department of pathology of cornea, under the guidance of the head of the department, DMedSc, Prof. G. Drozhzhyna, have developed an enhanced layered AMT technique. This technique involves creating a multilayer amniotic graft by folding it several times and then securing it with a single row of sutures to the edges of the corneal defect.

A clinical study was conducted at the corneal pathology department to evaluate the effectiveness of this enhanced AMT method in patients with corneal ulcers of various etiologies. As a result, a certificate of registration for the work titled “Advanced Technique of Multilayer Amniotic Membrane Transplantation” and a technology registration card (№ 0624U000013, dated January 12, 2024) were obtained.

The proposed AMT method allows for a 2-3 times reduction in the number of sutures on the cornea, thereby minimizing surgical trauma to the corneal surface. Additionally, the use of the enhanced technique promotes reduced inflammatory reactions and faster epithelialization of the corneal surface.

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